Phospho explorer antibody array

WebThe Human Phospho-Kinase Array (Catalog # ARY003B) is a rapid, sensitive, and economical tool to simultaneously detect the relative site-specific phosphorylation of 43 kinases and 2 related total proteins without performing numerous immunoprecipitations and Western blots. WebMay 6, 2014 · Several phosphokinase antibody arrays have been developed , including Phospho Explorer Array (Full Moon BioSystems), Signal Transduction AntibodyArray (Hypromatrix), Kinex Antibody Microarray (Kinexus Bioinformatics), Human Phospho-Kinase Antibody Array (R&D system), and Panorama Antibody Array XP725 (Sigma-Aldrich).

Cell Signalling - how is the Phospho Explorer antibody array used ...

WebSep 1, 2024 · Total RNA was extracted by chloroform and isopropanol precipitation. cDNA was obtained using the SuperScript III First-Strand Synthesis System (Invitrogen, 18080-051). qPCR analyses were performed with primers listed below using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725270) in at least duplicate in three … WebPhospho Explorer Array is a glass-based Array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples. It is a high-throughput ELISA based antibody microarray for qualitative/semi-quantitative protein … solihull windows and conservatories limited https://jessicabonzek.com

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WebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). WebThe antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. Learn more about cytokine … solihull window centre

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Category:Phosphoproteome Analysis of Invasion and Metastasis …

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Phospho explorer antibody array

Mechanism by which PF-3758309, a Pan Isoform Inhibitor of p21 …

Web2 Citations. Detects 55 phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types but intended for use with cell and tissue lysates. MANUAL / SDS. $901.00. Size. In stock. SKU. AAH-PPP-1-2. WebAKT Phospho-Specific Array includes 137 highly specific and well-characterized phosphorylation antibodies in the AKT pathway. Product detail AKT/PKB Signaling Phospho-Specific Array

Phospho explorer antibody array

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WebPhospho Explorer Antibody Array data of KGN cells treated with Hu‐17. (A) Heatmap of proteins differently phosphorylated proteins in KGN cells with Hu‐17 treatment. The data included in the heatmap is beyond cutoff line with the change fold is ≤77% or ≥130%. A is the control group, B is the Hu‐17 treated for 3 h group, C is the Hu ... WebWe assessed serum protein profiles of early parous, late parous, and nulliparous women using the Phospho Explorer antibody array. Significantly altered proteins identified were validated by ...

WebNov 14, 2024 · The Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and biological processes. WebFeb 28, 2024 · Building antibody arrays using capture reagents targeting proteins in the signalling cascade of interest has enabled researchers to investigate changes in protein profiles and modifications in signalling pathways in …

WebMoon BioSystems’ antibody arrays allow researchers to detect and analyze hundreds of proteins simultaneously on a single slide, saving precious resources and reducing the number of variables that affect experimental outcome. Our unique collection of antibody arrays includes phospho-specific arrays WebNov 11, 2024 · First, we used a previously described intrabody30 (Ib30)-based sensor for βarr1, which selectively recognizes receptor-bound conformation of βarr1 and reports agonist-induced formation of the receptor-βarr1 complex in cellular context ( Baidya et al., 2024a; Baidya et al., 2024b ).

WebPhospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and … Antibody Array Assay Kit is designed for easy and reliable processing of antibody … Chen P, Huang H, Bone marrow stromal cells protect acute myeloid leukemia … Signaling Explorer Antibody Array features 1358 unique antibodies covering 20 cell … Signaling Explorer Array: Garnett D, Caveolae as a target to quench …

WebMay 13, 2024 · Phosphodiesterase type 5 (PDE5) inhibitors are the most commonly used first-line treatment options for ED; however, these agents are ineffective in ~30% of patients and ultimately cannot rescue... solihull windows shirleyWebMar 18, 2024 · The Phospho Explorer Antibody Array kit used in this study was obtained from Full Moon BioSystems (catalog PEX100; Sunnyvale, CA, USA) and covered 1318 well-characterized antibodies, the phosphorylated and non-phosphorylated state of these proteins will be detected at the same time (Pulito et al. 2016 ). solihull windows and doorsWebApr 29, 2024 · Phosphoprotein profiling by the Phospho Explorer Antibody Array PEX100, which was designed and manufactured by Full Moon Biosystems, Inc. (Sunnyvale, CA), contains 1318 antibodies. Each of the antibodies has two replicates that are printed on coated glass microscope slide, along with multiple positive and negative controls. solihull women\u0027s aidWebApr 13, 2024 · We used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. small base desk chairWebTo understand the role of phosphoproteomic signaling in carvedilol's anticancer activity, the mouse epidermal JB6 P+ cells treated with EGF, carvedilol, or their combination were analyzed using the Phospho Explorer Antibody Array containing 1318 site-specific and phospho-specific antibodies of over 30 signaling pathways. solihull window cleaningWebApoptosis Phospho Antibody Array This array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study serine/threonine and tyrosine phosphorylation at specific sites. solihull window cleanersWebFeb 12, 2024 · Phospho-protein array Screening of 1318 phospho-proteins (Phospho Explorer Antibody Array #KAS02) was performed in hippocampal neurons under two experimental conditions: vehicle and 100 nM... solihull wizard