Gfp transgenic n. benthamiana 16c

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August undefined, 2024

WebSep 24, 2011 · The transgenic GFP-expressing N. benthamiana line 16c used in this study has been previously described . Seeds (kindly provided by Professor D. Baulcombe, Sainsbury Laboratory, Norwich, UK) were sown in small pots with an artificial potting mix (50% sand and 50% peat moss) in a plant growth chamber at 20/24°C (night/day), 50% … WebApr 28, 2016 · Transgenic N. benthamiana plants expressing green fluorescent protein (GFP, line 16c) were infiltrated with a mixture of Agrobacterium harboring 35S-GFP and …

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WebApr 16, 2010 · (A) Leaves of 16c GFP-transgenic plants were coinfiltrated with 35S-GFP and either empty vector, 35S-p19, 35S-myc-NtRDR1, 35S-NtΔRDR1, or 35S-NtRDR1m. Photographs were taken under UV light at 4 dpa. (B) RNA gel blot analysis of GFP mRNA and GFP -derived siRNA accumulation. 32 P-labeled GFP DNA or RNA probes were used. WebFeb 23, 2024 · Nicotiana benthamiana is employed around the world for many types of research and one transgenic line has been used more extensively than any other. This … items that contain tce

The widely used Nicotiana benthamiana 16c line has an …

WebApr 2, 2024 · The GFP fluorescence was observed in transiently transformed N. benthamiana leaves at 2 dpi using confocal microscope. Bar: 100 μm. D Quantification … WebJul 22, 2024 · Leaves of 16C transgenic N. benthamiana plants carrying GFP were infiltrated with a mixture of agrobacterium harboring 35S-GFP and either a test or a control vector (Fig. 5A). The pGD + GFP agrobacterium culture harboring empty vector was infiltrated into 16C plants as negative control and P19 + GFP agrobacterium culture … WebJan 4, 2024 · GFP expression was equivalent to 16C in a two-copy event. Local GFP silencing was observed in all transgenic and 16C hemizygous lines after topical application of delivery formulations... items that cost 1 million dollars

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WebApr 8, 2024 · The ability of LIYV vectors for VIGS was validated by silencing the endogenous gene PDS in Hc-Pro transgenic N. benthamiana plants, and the transgene GFP in N. benthamiana line 16c plants. The photo-bleaching phenotype was observed continuously in the veins of Hc-Pro N. benthamiana plants infected with LIYV vector … WebFeb 23, 2024 · benthamiana 16c are likely to be deeper and more accurate with a greater knowledge of the GFP gene integration site. Therefore, using next generation … items that cost 1 dollarWebMay 5, 2024 · In addition, ToLCGdV C4 suppresses systemic gene silencing in the transgenic N. benthamiana line 16c, but not local gene silencing induced by sense GFP in wild-type N. benthamiana plants. Moreover, C4 suppresses transcriptional gene silencing (TGS) by reducing the DNA methylation level of 35S promoter in 16c-TGS N. … items that contain cfcs

"WebIn this study, we identified a new citrus vein enation virus (CVEV) isolate (named CVEV-DT1) through sRNA high-throughput sequencing and traditional sequencing. Phylogenetic analysis based on whole genome sequences of all known CVEV isolates revealed that CVEV-DT1 was in an evolutionary branch with other isolates from China. Molecular … " - Gfp transgenic n. benthamiana 16c

Gfp transgenic n. benthamiana 16c

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WebGreen fluorescent protein (GFP) has been widely used as a visual reporter to study RNA silencing and viral-mediated suppression of RNA silencing in the model plant Nicotiana benthamiana. In transgenic N. benthamiana plants expressing an endoplasmic reticulum targeted GFP variant (16c) known as mGFP5, RNA silencing can be induced by ectopic … WebLocalization of p11 in Nicotiana benthamiana and 16c epidermal cells. (A) Localization of pΔGUS‐GFP (GFP, green fluorescent protein) in N. benthamiana (control). (B) Localization of p11‐GFP in N. benthamiana. The enlarged zone reveals that the fluorescence forms a network and that the green fluorescent granules exist on it.

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WebMar 1, 2024 · Green fluorescent protein (GFP) transgenic N. benthamiana plants (line 16c) [11] was used in the present study and grown from seeds in a growth chamber under 16 h light and 8 h dark photoperiod at 25 °C. Healthy leaves of six-week-old plants were used for agroinfiltration experiment. WebDec 16, 2024 · Leaves and buds of Nb-16C plants have been sprayed with 200 μl of dsRNA-midGFP at four different concentrations (10, 20, 200, and 240 ng/μl). In addition, 200 μl of dsRNA-5′GFP has also been sprayed on Nb-16C plants leaves and buds at three different concentrations (24, 48, and 240 ng/μl).

WebGFPtransgenic plants co-agro-inoculated with BaMV and satBaMV vectors carrying sulphur and GFPgenes, respectively, could simultaneously silence both genes. Moreover, the silenced plants could still survive with the silencing of genes essential for plant development such as heat-shock protein 90 (Hsp90)and Hsp70. WebOct 2, 2006 · To create a similar model for studies of glial cells in zebrafish, we have generated transgenic lines of zebrafish, Tg ( gfap: GFP), that express GFP driven by …

WebMar 15, 2024 · The GFP16C-like systemic silencing phenotype was only observed in the two-copy line. The partial transposase had no impact on transgene expression level, … WebDec 15, 2024 · The 16c line transgenic N. benthamiana plants carrying a GFP transgene have been used extensively to study RNA silencing and VSR . The plants of this …

WebIn this study, transgenic N. benthamiana (line 16c), which shows strong constitutive expression of the green fluorescent protein (GFP) (Brigneti et al. , 1998) and allows non-disruptive...

Web3.1.1 Structure of GFP. The green fluorescent protein is a small protein (28 kDa), with a barrel-like structure composed of 11 β-sheets with a central α-helix running up the axis of … items that contain leadWebtransgenic line has been used more extensively than any other. This line, 16c, expresses the Aequorea victoria green fluorescent protein (GFP), highly and constitutively, and … items that contain bpaWebJan 14, 2024 · When we inoculated the green fluorescent protein (GFP)-expressing transgenic Nicotiana benthamiana (16c) with the CMV-A1 constructs containing various sizes of the 35S promoter sequences, we found that the GFP expression levels were downregulated due to RdDM and that the sizes of the virus-integrated sequences were … items that contain high fructose corn syrupWebJun 29, 2024 · Nicotiana benthamiana 16c plants were rub-inoculated with the viral sap of TRV-35S, TRV-GFP or TRV-WT. After rub inoculation, plants were kept at different temperatures (15, 22 and 29°C) and were monitored at regular intervals under UV light. Fig. 1 Open in figure viewer PowerPoint items that contain palladiumWebOct 1, 2024 · In this study, we found that the C4 protein encoded by tomato leaf curl Yunnan virus (TLCYnV) suppresses methylation of the viral genome through interacting with and impairing the DNA-binding ability of NbDRM2, a pivotal … items that come in pairsWebJan 4, 2024 · GFPexpression was equivalent to 16C in a two-copy event. Local GFPsilencing was observed in 41 all transgenic and 16C hemizygous lines after topical … items that contain caffeineWebFeb 6, 2014 · Here we report that the Nicotiana benthamiana calmodulin-like protein Nbrgs-CaM is required for the functions of the VSR βC1, the sole protein encoded by the DNA satellite associated with the geminivirus Tomato yellow leaf curl China virus (TYLCCNV). Nbrgs-CaM expression is up-regulated by the βC1. items that contain palm oil