Biotin wga
WebJul 15, 2024 · Passivation was carried out by adding 3% BSA solution into each well and incubated for 30 min at 37 °C in order to block the non-specific binding sites on the … WebMar 27, 2012 · The membrane was then incubated at room temperature for 1 h in solution containing 0.8 µg/ml wheat germ agglutinin conjugated with biotin (WGA-biotin) (Sigma-Aldrich). After washing four times with PBS, PIA was detected using horseradish peroxidase-conjugated streptavidin followed by chemiluminescence detection (Pierce).
Biotin wga
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WebAug 19, 2024 · Biotin (vitamin B7) is a vitamin found in foods like eggs, milk, and bananas. Biotin deficiency can cause thinning of the hair and a rash on the face. Biotin is an … WebMar 8, 2024 · As WGA-HRP consistently outperformed DNA-APEX2 by proteomics and represents a more facile method amenable to broad application in the field, we chose to …
WebDec 2, 2024 · Recently, proximity based biotin identification (BioID) has emerged as an alternative approach to traditional co-immunoprecipitation. In this protocol, a mutant biotin ligase promiscuously labels proximal binding partners with biotin, and resulting biotinylated proteins are purified using streptavidin conjugated beads.
WebThis product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. Handling Advice. Aliquot to avoid repeated freezing and thawing. Storage. -20 °C. Storage Comment. Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. WebFeb 1, 1986 · Fig. 2a and b. Double-labeled neurons in the lateral superior olivary nucleus after injection of biotin-WGA (b-WGA) into the cochlea and incubation with antiserum against CGRP. Ipsilateral side of the injection, a b-WGA labeled cells made visible by streptavidin-Texas red. b CGRPI neurons labeled with FITC in the same field as a.
WebTechnical Information. Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. This lectin …
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