WebThe conjugate between biotin–BMCC (Fig. 1A) and GSH was analyzed with an ABI 3000 electrospray mass spectrometer (Applied Biosystems, Foster City, CA) in positive ion mode. A 20 μM sample of BMCC–GSH conjugate was infused into the mass spectrometer, using a Harvard syringe pump, at a flow rate of 20 μl/min. WebBiotin-BMCC is a maliemide conjugated biotin molecule that has a high affinity for free thiol groups of cysteines ( Figure 1). While performing step 4.2, prepare 0.5 ml of Biotin …

A Structural Hypothesis for the Transition between …

WebSep 4, 2003 · The sulfhydryl-reactive reagent, BMCC (Pierce Endogen) was used to probe reaction with Cys substitutions at positions in the N-terminal region of BtuB (residues 1–17 and 25–31) and to detect changes in labeling in the presence of BtuB transport substrates. BMCC contains a maleimide moiety joined to biotin through a cyclohexyl-butyl linker. WebMar 5, 2024 · Biotin-BMCC will bind the previously S-acylated protein residues and the resulting lysates can be processed by western blotting. The level of protein palmitoylation is detected with streptavidin ... shuck me hochatown

In vitro Detection of S-acylation on Recombinant Proteins via the ...

WebJun 1, 2007 · Since biotin-BMCC was more accessible to the site between C H1 and C L (Site 1) than that between C H2 and C H2 at the hinge region (Site 2; Fig. 2, Condition A), using a mixture of biotin derivative and biotin-free maleimide could result in a competitive reaction at Site 1.The accessibility of free maleimide would potentially be superior to that … WebProtein S-palmitoylation is a reversible post-translational lipidation in which palmitic acid (16:0) is added to protein cysteine residue by a covalent thioester bond. This modification plays an active role in membrane targeting of soluble proteins, protein–protein interaction, protein trafficking, and subcellular localization. Moreover, palmitoylation is … WebFeb 18, 2013 · Biotin-BMCC is a maliemide conjugated biotin molecule that has a high affinity for free thiol groups of cysteines (Figure 1). While performing step 4.2, prepare … shuck memorial baptist church lewisburg wv